DOSE-DEPENDENT CHANGES IN BIOMARKERS OF OXIDATIVE STRESS IN HUMAN ERYTHROCYTES FOLLOWING IN VITRO TREATMENT WITH EXTRACTS FROM BERRIES OF EUROPEAN MISTLETOE (VISCUM ALBUM L.)
DOI:
https://doi.org/10.32782/naturaljournal.7.2024.1Keywords:
European mistletoe (Viscum album L.), human blood, extracts, 2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives of oxidative modification of proteins, total antioxidant capacity (TAC)Abstract
Radical scavenging activity and protective effects against oxidative stress caused by free radicals, nitric oxide and superoxide anion have been demonstrated for a number of mistletoe extracts and isolated lectins. The aim of the present study was to determine the antioxidant activity of extracts from the berries of mistletoe (Viscum album L.). For this purpose, biomarkers of oxidative stress [2-thiobarbituric acid reactive substances (TBARS) as a biomarker of lipid peroxidation, carbonyl derivatives of oxidative modification of proteins, total antioxidant capacity (TAC)] were used in human blood after in vitro incubation with extracts derived from mistletoe berries at two final concentrations (5 and 2.5 mg/mL). The results of our study showed that treatment with mistletoe berry extracts resulted in a significant increase in TBARS levels in human erythrocytes after in vitro treatment with extracts at final concentrations of 5 mg/mL compared to untreated samples. On the other hand, a statistically non-significant decrease in TBARS levels was observed for the extract at a final concentration of 2.5 mg/ mL. The levels of aldehydic derivatives of oxidatively modified proteins were statistically significantly increased in samples treated in vitro with mistletoe berry extracts at a final concentration of 5 mg/mL compared to untreated samples, and this increase was statistically significant. Treatment of human erythrocytes with mistletoe berry extracts at a final concentration of 2.5 mg/mL resulted in a statistically significant decrease in the levels of aldehydic derivatives of oxidatively modified proteins. The reduction was 24.1% (p < 0.05). When human erythrocytes were incubated with mistletoe berry extracts, the levels of ketonic derivatives of OMP were at the same level as in untreated samples. Treatment of human erythrocytes with mistletoe berry extracts at a final concentration of 2.5 mg/ml resulted in a statistically non-significant decrease in the levels of ketonic derivatives of oxidatively modified proteins. TAC levels in human erythrocytes were increased after in vitro incubation with mistletoe berry extracts (final concentration of 5 mg/mL) compared to untreated samples. This represented a 29% (p < 0.05) increase in TAC levels compared to untreated samples. TAC levels in human erythrocytes after in vitro incubation with mistletoe berry extracts (final concentration 2.5 mg/mL) were at the same level as in untreated samples. Future studies can add to the current findings to better understand the antioxidant properties of mistletoe berry extracts, with the potential to develop treatments and products using these extracts.
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