OPTIMIZATION OF CULTURE MEDIA FOR INDUSTRIAL CULTIVATION OF THE RECOMBINANT STRAIN ESCHERICHIA COLI BL21
DOI:
https://doi.org/10.32782/naturaljournal.9.2024.2Keywords:
Escherichia coli BL21, media optimization, alternative carbon source, alternative nitrogen source, recombinant proteinAbstract
This study presents a comprehensive analysis of scientific literature published between 2019 and 2024, indexed in Web of Science and Scopus databases. The review focuses on identifying optimization strategies for culture media to enhance the industrial cultivation of Escherichia coli BL21 strain for the production of recombinant proteins. This strain is widely used in industry due to its lack of certain proteases, making it ideal for producing stable protein products. The research highlights key factors influencing protein expression and biomass growth, including carbon and nitrogen sources, trace elements, additional components, and pH levels. Altering these key factors can increase cell yield and product quality. The analysis revealed that optimizing the culture medium composition through the use of alternative carbon and nitrogen sources can significantly improve bacterial cell growth and impact the quantity and quality of the recombinant protein. Alcohols such as mannitol and glycerol, sugars like lactose, as well as sugar-containing by-products from the food industry can be used as alternative carbon sources (blackstrap molasses, corn-steep liquor and whey). Additionally, complex compounds like lignocellulose can be utilized. Many alternative carbon sources can also provide nitrogen. The use of alternative carbon and nitrogen sources, on the one hand, can reduce the cost of recombinant protein production and thus affect bioeconomy, but on the other hand, can influence metabolic pathways for the assimilation of other elements and alter the duration of growth phases, which is crucial for industrial microbial cultivation. Optimization of the culture medium has complex consequences, and this process should be considered holistically.
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