STUDY OF MORPHOLOGICAL FEATURES OF THE SEROUS COLUMN OF THE MESSIAH OF THE COLON INTESTINAL

Authors

DOI:

https://doi.org/10.32782/naturaljournal.11.2025.8

Keywords:

intestine, film preparations, lymphocytes, abdominal cavity, intestinal mesentery, gastrointestinal tract

Abstract

Modern research emphasizes the growing role of applied morphology in studying the causesand mechanisms of formation of anatomical variants of the structure of internal organs. The relevance of this work lies in the need for a comprehensive analysis of the topographic and anatomicalfeatures of the serous membrane of the large intestine, which is important for creating new, more effective methods of surgical correction. To study the morphological features of the serous membrane of the mesentery of the large intestine and the features of taking film material from white rats inthe norm for further histological studies. The study was conducted on 10 young male white rats,aged 5,0–6,0 months and weighing 150–200 g. Macroscopic examination provided for determiningthe shape of the mesentery of the large intestine and its morphometric characteristics, in particular length, width and area. Fixation of the preparations was carried out in Bouin’s solution for 24 hours, after which the samples were washed in running water for 2 hours. Staining was performed according to the standard method using hematoxylin and eosin. Ready-made stained preparations were fixed in Canadian balsam and examined at different magnifications of the microscope. It was established that the mesentery of the large intestine is a set of dense folds of the peritoneum, covering the loops of the large intestine, separating them from the posterior wall of the abdominal cavity. The mesentery has a thin, elastic, smooth, almost transparent, shiny structure, of uniform density, in which the blood vessels are located closer to the cavity of the large intestine. During the planimetric analysis, the length of the mesentery of the large intestine was examined – 11,2 ± 0,3 cm; width – 6,9 ± 0,8 cm; area – 57,4 ± 0,4 cm2. It was established that the number of lymphocytes per standard studied areaof the serous membrane of the colon mesentery is 4,8 ± 0,42 cells (1 000 μm2), which is withinnormal limits.

References

Кущ О.Г. Виявлення В-лімфоцитів у плаценті при резус-ізоімунному конфлікті матері та плоду. Вісник морфології. 2007. № 13 (2). С. 290–293.

Пайдаркіна А.П., Кущ О.Г. Дослідження морфологічних особливостей очеревини білих щурів й методика її забору. Морфологія. 2023. № 3 (17). C. 162–167. https://doi.org/10.26641/1997-9665.2023.3.163-167.

Пайдаркіна А.П., Кущ О.Г. Морфофункціональні зміни очеревини і її структур при спайковій хворобі. Вісник проблем біології і медицини. 2024a. № 1 (172). С. 97–106. https://doi.org/10.29254/2077-4214-2024-1-172-97-106.

Пайдаркіна А.П., Кущ О.Г. Ремоделювання очеревини і зміни її лімфоїдного компоненту при експериментальному моделюванні спайкової хвороби у щурів. ScienceRise: Biological Science, 2024b. № 4 (37). С. 10–16. https://doi.org/10.15587/2519-8025.2024.301278.

Avwioro G. Histochemical Uses of Haematoxylin – A Review. JPCS. 2011. Vol. 1. P. 24–34. https://doi.org/10.1111/iej.12859.

Bunni J., Coffey J.C., Kalady M.F. Resectional surgery for malignant disease of abdominal diges-tive organs is not surgery of the organ itself, but also that of the mesenteric organ. Tech Coloproctol. 2020. № 24. P. 757–760. https://doi.org/10.1007/s10151-020-02197-7.

Byrnes K.G., Walsh D., Walsh L.G. The development and structure of the mesentery. Commun Biol. 2021. № 4. P. 982–985. https://doi.org/10.1038/s42003-021-02496-1.

Coffey J.C., Dockery P. Peritoneum, mesentery and peritoneal cavity. Gray’s Anatomy: The Anatomical Basis of Clinical Practice. 2020. 42nd edition. P. 1150–1160.

Daisuke S., Ji H.K, Shunichi S., Gen M., José F.R. Topographical anatomy of the greater omen-tum and transverse mesocolon: a study using human fetuses. Anatomy and Cell Biology. 2019. № 5. P. 443–454. https://doi.org/10.5115/acb.19.112.

Felix M.D. Observation on the surface cells of the mouse omentum as studied with the Phasecontrast and Electron Microscopes. Journal of the National Cancer Institute. 1961. № 27. Р. 713–745.

Jackson-Jones L.H., Bénézech C. FALC stromal cells define a unique immunological niche for the surveillance of serous cavities. Curr. Opin. Immunol. 2020. № 64. Р. 42–49. https://doi. org/10.1016/j.coi.2020.03.008.

Kai Y. Intestinal villus structure contributes to even shedding of epithelial cells. Biophysical Journal. 2021. № 120. Р. 699–710. https://doi.org/10.1016/j.bpj.2021.01.003.

Krishnan V., Tallapragada S., Schaar B., Kamat K., Chanana A.M., Zhang Y. Omental macro-phages secrete chemokine ligands that promote ovarian cancer colonization of the omentum via CCR. Communications Biology. 2020. № 223. Р. 524–529. https://doi.org/10.1038/s42003-020-01246-z.

Kuper C.F., Pieters R.H., van Bilsen J.M. Nanomaterials and the Serosal Immune System in the Thoracic and Peritoneal Cavities. Int. J. Mol. Sci. 2021. № 22. Р. 2610–2618. https://doi.org/10.3390/ijms22052610.

Murando F., Peloso A., Cobianchi L. Experimental Abdominal Sepsis: Sticking to an Awkward but Still Useful Translational Model. Mediators of Inflammation. 2019. № 3. Р. 8–10. https://doi.org/10.1155/2019/8971036.

O’Regan P.W., Mhuircheartaigh J.N., Scanlon T.G., Shelly M.J. Radiology of the Mesentery. Clin Colon Rectal Surg. 2022. № 35. Р. 328–337. https://doi.org/10.1055/s-0042-1744481.

Schurink B., Cleypool C.J., Bleys R.L. A rapid and simple method for visualizing milky spots in large fixed tissue samples of the human greater omentum. Biotechnic & Histochemistry. 2019. № 94. Р. 429–434. https://doi.org/10.1080/10520295.2019.1583375.

Vdoviaková K., Petrovová E., Maloveská M., Krešáková L., Teleky J., Elias M.Z., Petrášová D. Surgical Anatomy of the Gastrointestinal Tract and Its Vasculature in the Laboratory Rat. Gastroenterol Res Pract. 2016. № 26. Р. 32–36. https://doi.org/10.1155/2016/2632368.

Wang Q., Huang Y., Zhou R., Wu K., Li W., Shi L., Xia Z., Tao K., Wang G. Regulation and func-tion of IL-22 in peritoneal adhesion formation after abdominal surgery. Wound Repair Regen. 2020. № 28. Р. 105–117. https://doi.org/10.1111/wrr.12740.

Published

2025-03-28